1GSE

GLUTATHIONE TRANSFERASE A1-1 COMPLEXED WITH AN ETHACRYNIC ACID GLUTATHIONE CONJUGATE (MUTANT R15K)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-01-20
Detector	RIGAKU RAXIS
Spacegroup name	C 1 2 1
Unit cell lengths	100.400, 94.800, 51.800
Unit cell angles	90.00, 92.96, 90.00

Refinement procedure

Resolution	7.500 - 2.000
R-factor	0.197
Rwork	0.197
R-free	0.24400 *
RMSD bond length	0.009
RMSD bond angle	1.440 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.000
Rmerge	0.073
Total number of observations	64609 *
Number of reflections	30538
Completeness [%]	93.4
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	55 (mg/ml)
2	1	drop	PEG2000	10-20 (%)
3	1	drop	Tris-HCl	100 (mM)
4	1	drop	NaAc	30 (mM)
5	1	drop	beta-mercaptoethanol	1 (%)
6	1	reservoir	PEG2000	10-20 (%)
7	1	reservoir	Tris-HCl	100 (mM)
8	1	reservoir	NaAc	30 (mM)
9	1	reservoir	beta-mercaptoethanol	1 (%)