1GQ2
Malic Enzyme from Pigeon Liver
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 277 |
Collection date | 2001-02-15 |
Detector | ADSC Q-4 |
Spacegroup name | P 1 |
Unit cell lengths | 124.149, 140.863, 167.079 |
Unit cell angles | 90.05, 87.16, 75.63 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.211 * |
Rwork | 0.210 |
R-free | 0.25800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB |
RMSD bond length | 0.008 |
RMSD bond angle | 22.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.082 | 0.214 |
Total number of observations | 570674 * | |
Number of reflections | 314771 * | |
<I/σ(I)> | 10.6 | 3.6 |
Completeness [%] | 83.0 * | 76 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | 4 * | 100 MM SODIUM CITRATE, PH 5.5, 8% PEG6000, 1M LICL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | pigeon ME | 10 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
3 | 1 | drop | oxalate | 10 (mM) | |
4 | 1 | drop | 5 (mM) | ||
5 | 1 | drop | NADP+ | 0.23 (mM) | |
6 | 1 | drop | dithiothreitol | 2 (mM) | |
7 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
8 | 1 | reservoir | PEG6000 | 8 (%) | |
9 | 1 | reservoir | 1 (M) |