1GP9
A New Crystal Form of the Nk1 Splice Variant of Hgf/Sf Demonstrates Extensive Hinge Movement and Suggests that the Nk1 Dimer Originates by Domain Swapping
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-03 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.480, 78.260, 304.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.500 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1NK1 1BHT |
RMSD bond length | 0.011 |
RMSD bond angle | 24.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.500 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.045 | 0.352 |
Number of reflections | 33095 | |
<I/σ(I)> | 12 | |
Completeness [%] | 92.2 | 95.8 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * | 18% PEG4000, 10% 2-PROPANOL, 0.1M SODIUM HEPES, PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MES | 0.05 (M) | |
2 | 1 | drop | 0.1 (M) | pH6.0 | |
3 | 1 | drop | protein | 25-35 (mg/ml) | |
4 | 1 | reservoir | 2-propanol | 10 (%) | |
5 | 1 | reservoir | PEG4000 | 18 (%) | |
6 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |