1GP9

A New Crystal Form of the Nk1 Splice Variant of Hgf/Sf Demonstrates Extensive Hinge Movement and Suggests that the Nk1 Dimer Originates by Domain Swapping

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-09-03
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	73.480, 78.260, 304.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.500
R-factor	0.237
Rwork	0.237
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1NK1 1BHT
RMSD bond length	0.011
RMSD bond angle	24.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.500
High resolution limit [Å]	2.440	2.440
Rmerge	0.045	0.352
Number of reflections	33095
<I/σ(I)>	12
Completeness [%]	92.2	95.8
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *	18% PEG4000, 10% 2-PROPANOL, 0.1M SODIUM HEPES, PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MES	0.05 (M)
2	1	drop		0.1 (M)	pH6.0
3	1	drop	protein	25-35 (mg/ml)
4	1	reservoir	2-propanol	10 (%)
5	1	reservoir	PEG4000	18 (%)
6	1	reservoir	sodium HEPES	0.1 (M)	pH7.5