1GP9
A New Crystal Form of the Nk1 Splice Variant of Hgf/Sf Demonstrates Extensive Hinge Movement and Suggests that the Nk1 Dimer Originates by Domain Swapping
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.480, 78.260, 304.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.500 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1NK1 1BHT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.045 | 0.352 |
| Number of reflections | 33095 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 92.2 | 95.8 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 20 * | 18% PEG4000, 10% 2-PROPANOL, 0.1M SODIUM HEPES, PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 0.05 (M) | |
| 2 | 1 | drop | 0.1 (M) | pH6.0 | |
| 3 | 1 | drop | protein | 25-35 (mg/ml) | |
| 4 | 1 | reservoir | 2-propanol | 10 (%) | |
| 5 | 1 | reservoir | PEG4000 | 18 (%) | |
| 6 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
