1GA5
CRYSTAL STRUCTURE OF THE ORPHAN NUCLEAR RECEPTOR REV-ERB(ALPHA) DNA-BINDING DOMAIN BOUND TO ITS COGNATE RESPONSE ELEMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9054 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.920, 52.020, 78.880 |
| Unit cell angles | 85.84, 76.61, 74.48 |
Refinement procedure
| Resolution | 19.600 - 2.400 |
| R-factor | 0.253 * |
| Rwork | 0.253 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a6y residues 132-198 from chain A & B plus DNA |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.083 | 0.323 * |
| Number of reflections | 25370 | 2476 * |
| <I/σ(I)> | 6.8 | 2.5 |
| Completeness [%] | 87.3 * | 82.8 * |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 17 * | Zhao, Q., (1998) Mol. Cell, 1, 849. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG8000 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | Tris |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.6 (mM) | |
| 2 | 1 | drop | protein | 1.2 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 20-25 (%) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | 400 (mM) | ||
| 6 | 1 | reservoir | Tris | pH7.5 |
