1G3N
STRUCTURE OF A P18(INK4C)-CDK6-K-CYCLIN TERNARY COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.780, 146.950, 164.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.228 |
| Rwork | 0.224 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.760 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.057 | 0.299 |
| Total number of observations | 131920 * | |
| Number of reflections | 40272 | |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 95.0 | 91.9 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | (NH4)2SO4, Dioxane, Tris-HCl, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 40 (mM) | ||
| 10 | 1 | reservoir | Tris-Cl | 100 (mM) | |
| 2 | 1 | drop | dithiothreitol | 5 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | ATP | 0.5 (mM) | |
| 5 | 1 | drop | Tris-Cl | 25 (mM) | |
| 6 | 1 | drop | protein | 1.0 (mg/ml) | |
| 7 | 1 | reservoir | ammonium sulfate | 38 (%sat) | |
| 8 | 1 | reservoir | dioxane | 6 (%(v/v)) | |
| 9 | 1 | reservoir | dithiothreitol | 5 (mM) |
