1FZV
THE CRYSTAL STRUCTURE OF HUMAN PLACENTA GROWTH FACTOR-1 (PLGF-1), AN ANGIOGENIC PROTEIN AT 2.0A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 62.600, 62.600, 84.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.170 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24700 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.364 |
| Total number of observations | 161044 * | |
| Number of reflections | 20658 | |
| <I/σ(I)> | 19.67 | |
| Completeness [%] | 94.1 | 98.8 * |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | MES, MPD and Calcium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 16K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | MES | 0.05 (M) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | MPD | 7.5 (%(v/v)) | |
| 5 | 1 | reservoir | MES | 0.1 (M) | |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | MPD | 15 (%(v/v)) |
