1FZ5
METHANE MONOOXYGENASE HYDROXYLASE, FORM II CRYSTALLIZED ANAEROBICALLY FROM REDUCED ENZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.240, 171.570, 220.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.400 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.25300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.470 |
| Total number of observations | 907358 * | |
| Number of reflections | 104366 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 97.9 | 97.3 |
| Redundancy | 8.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8000, CaCl2, MOPS, 1,10-decanedioic acid. pH 7.0, VAPOR DIFFUSION, HANGING DROP at 298 K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 6 (%(w/w)) | |
| 10 | 1 | drop | sodium azide | 0.005 (%) | |
| 2 | 1 | reservoir | MOPS | 50 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | sodium azide | 0.01 (%) | |
| 5 | 1 | drop | MMOH | 25 (mM) | |
| 6 | 1 | drop | 1,10-decanedioic acid | 12.5 (%sat) | in H2O |
| 7 | 1 | drop | PEG8000 | 3 (%) | |
| 8 | 1 | drop | MOPS | 25 (mM) | |
| 9 | 1 | drop | 110 (mM) |
