1FS2
INSIGHTS INTO SCF UBIQUITIN LIGASES FROM THE STRUCTURE OF THE SKP1-SKP2 COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.670, 87.020, 178.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
Rwork | 0.246 |
R-free | 0.28700 |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.980 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.900 * | |
Rmerge | 0.085 * | 0.388 * |
Total number of observations | 178486 * | |
Number of reflections | 23509 * | |
Completeness [%] | 96.9 * | 98.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | 4 * | microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 16-18 (%) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | reservoir | dithiothreitol | 5 (mM) |