1FS1

INSIGHTS INTO SCF UBIQUITIN LIGASES FROM THE STRUCTURE OF THE SKP1-SKP2 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Spacegroup name	P 1 21 1
Unit cell lengths	46.540, 41.600, 87.230
Unit cell angles	90.00, 93.42, 90.00

Refinement procedure

Resolution	8.000 - 1.800
Rwork	0.218
R-free	0.27400
RMSD bond length	0.015 *
RMSD bond angle	1.890 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *
Rmerge	0.037 *	0.124 *
Total number of observations	206639 *
Number of reflections	29068 *
Completeness [%]	84.4 *	47.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	25 *	PEG 1500, sodium acetate, ammonium acetate, DTT, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG1500	36-38 (%)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	ammonium acetate	0.2 (M)
4	1	reservoir	dithiothreitol	5 (mM)