1FPM

MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	RIGAKU RAXIS
Spacegroup name	H 3 2
Unit cell lengths	160.876, 160.876, 256.118
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.000
R-factor	0.266
Rwork	0.266
R-free	0.31500 *
RMSD bond length	0.010 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.100
High resolution limit [Å]	3.000	2.990
Rmerge	0.088	0.182
Total number of observations	137437 *
Number of reflections	25025
Completeness [%]	97.0	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.6	295	AMMONIUM SULFATE, PEG 1000, POTASSIUM MALEATE, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	46 (%sat)
2	1	reservoir	dithiothreitol	1 (mM)
3	1	reservoir	PEG1000	1 (%)
4	1	reservoir	potassium maleate	50 (mM)	pH7.6