1FJL

HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	C 2 2 21
Unit cell lengths	72.210, 146.890, 77.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.000
R-factor	0.197 *
Rwork	0.198
R-free	0.27000 *
RMSD bond length	0.018
RMSD bond angle	2.800
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.058 *	0.185 *
Total number of observations	94960 *
Number of reflections	28315 *
Completeness [%]	92.9 *	83.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Na acetate	100 (mM)
2	1	1		20 (mM)
3	1	1	PEG1000	19 (%(w/v))
4	1	1	ethylene glycol	13 (%)	2 % increments over 30 min.