1FHW
Structure of the pleckstrin homology domain from GRP1 in complex with inositol(1,3,4,5,6)pentakisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-10 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.970, 72.220, 46.900 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 1.900 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27000 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.080 |
| Total number of observations | 53932 * | |
| Number of reflections | 18374 | |
| <I/σ(I)> | 20 | 10.7 |
| Completeness [%] | 95.2 | 80.5 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 21 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 2-10 (%) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 4 | 1 | drop | protein | 15 (mg/ml) | |
| 5 | 1 | drop | MES | 10 (mM) | |
| 6 | 1 | drop | 100 (mM) |
