1FE8

CRYSTAL STRUCTURE OF THE VON WILLEBRAND FACTOR A3 DOMAIN IN COMPLEX WITH A FAB FRAGMENT OF IGG RU5 THAT INHIBITS COLLAGEN BINDING

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-14
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	121.800, 183.600, 131.800
Unit cell angles	90.00, 116.20, 90.00

Refinement procedure

Resolution	29.900 - 2.030
R-factor	0.229
Rwork	0.227
R-free	0.26400
RMSD bond length	0.007
RMSD bond angle	1.410
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900	2.100
High resolution limit [Å]	2.030	2.030
Rmerge	0.061	0.404
Number of reflections	143255 *
<I/σ(I)>	20.4
Completeness [%]	85.9	51.5
Redundancy	4.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3 *	4 *	iso-propanol, MPD, cacodylate, sodium chloride, Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	isopropanol	13 (%(v/v))	precipitant
3	1	reservoir	MPD	22 (%(v/v))	precipitant
4	1	reservoir	cacodylic acid	100 (mM)