1FDJ
FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE FROM RABBIT LIVER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-11-10 |
| Detector | XENTRONICS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 377.250, 130.540, 80.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.100 |
| R-factor | 0.174 |
| Rwork | 0.171 |
| R-free | 0.21400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.770 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.250 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.092 | 0.180 |
| Number of reflections | 85613 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 85.1 | 68.1 |
| Redundancy | 4.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 45% saturated ammonium sulphate solution, 100mM Tris.HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
