1FAP
THE STRUCTURE OF THE IMMUNOPHILIN-IMMUNOSUPPRESSANT FKBP12-RAPAMYCIN COMPLEX INTERACTING WITH HUMAN FRAP
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-07-29 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.630, 52.140, 102.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.29900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.480 |
| Data reduction software | UCSD |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.071 |
| Total number of observations | 43447 * |
| Number of reflections | 6920 |
| Completeness [%] | 98.5 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | FKBP12 | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 60 (mM) | |
| 3 | 1 | drop | rapamycin | ||
| 4 | 1 | drop | methanol | ||
| 5 | 1 | drop | FRB | 10 (mg/ml) | |
| 6 | 1 | reservoir | PEG8000 | 200 (%(w/v)) | |
| 7 | 1 | reservoir | methylpentanediol | 10 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 10 (mM) |
