1F2X

STRUCTURE OF THE SINGLE-DOMAIN CAMELID ANTIBODY CAB-CA05

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-01-05
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	29.980, 43.860, 87.950
Unit cell angles	90.00, 93.23, 90.00

Refinement procedure

Resolution	31.000 - 2.100
R-factor	0.19
Rwork	0.190
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bzq
RMSD bond length	0.009
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.103	0.227
Number of reflections	13256
<I/σ(I)>	4.9	2.7
Completeness [%]	99.8	99.8
Redundancy	5.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4 *	293	Desmyter, A., (1996) Nature Struct. Biol., 3, 803. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein complex	5 (mg/ml)
2	1	drop	PEG8000	15-20 (%(w/v))
3	1	drop	potassium phosphate	0.1 (M)