1F07

STRUCTURE OF COENZYME F420 DEPENDENT TETRAHYDROMETHANOPTERIN REDUCTASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-07
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	162.800, 128.200, 109.200
Unit cell angles	90.00, 131.40, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.209
Rwork	0.208
R-free	0.23700
RMSD bond length	0.008
RMSD bond angle	21.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.020
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	0.290
Number of reflections	107409
<I/σ(I)>	14
Completeness [%]	95.0	88
Redundancy	3.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6	277	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	30 (%)
2	1	reservoir		0.1 (M)
3	1	reservoir		0.02 (M)