1EZV

STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	277
Detector technology	CCD
Collection date	1999-02-01
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	214.470, 163.920, 147.270
Unit cell angles	90.00, 117.50, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.253
Rwork	0.222
R-free	0.25400
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	15.000
High resolution limit [Å]	2.300	2.300
Rmerge	0.065	0.390
Total number of observations	1057968 *
Number of reflections	168625 *
<I/σ(I)>	12.4
Completeness [%]	84.9	73.9
Redundancy	6.27	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	277	5 % PEG 4000, 100 mM Tris, 0.05 % Undecyl-maltoside, 1 micromolar stigmatellin, pH 8.0, microseeding, temperature 277K
1	Vapor diffusion *	8	277	5 % PEG 4000, 100 mM Tris, 0.05 % Undecyl-maltoside, 1 micromolar stigmatellin, pH 8.0, microseeding, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein
2	1	drop	PEG4000
3	1	drop	Tris-HCl	100 (mM)
4	1	drop	UDM	0.05 (%)
5	1	reservoir	PEG4000	5-6 (%)