1EXQ
CRYSTAL STRUCTURE OF THE HIV-1 INTEGRASE CATALYTIC CORE DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 48.886, 48.886, 103.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.110 - 1.600 |
| R-factor | 0.228 * |
| Rwork | 0.253 |
| R-free | 0.27100 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.100 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | |
| Total number of observations | 213312 * | |
| Number of reflections | 35599 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 97.2 | 95.9 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | ammonium sulfate, cadmium chloride, Na citrate, DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 3 (mM) | |
| 4 | 1 | drop | protein | 6-10 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 6 | 1 | reservoir | 50 (mM) | ||
| 7 | 1 | reservoir | sodium citrate | 150 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
