1EXP

BETA-1,4-GLYCANASE CEX-CD

Experimental procedure

Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 41 21 2
Unit cell lengths	88.172, 88.172, 81.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.208
Rwork	0.208
R-free	0.28300
RMSD bond length	0.014
RMSD bond angle	22.400 *
Data reduction software	SDMS (DETECTOR SYSTEM (NIELSEN))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	1.800
Rmerge	0.041
Total number of observations	139457 *
Number of reflections	29464 *
Completeness [%]	99.0
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *		Bedarkar, S., (1992) J.Mol. Biol, 228, 693. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Cex	65 (mg/ml)	in water
2	1	drop	PEG4000	7.5-10 (%)
3	1	drop	sodium acetate	0.1 (M)
4	1	reservoir	PEG4000	7.5-10 (%)
5	1	reservoir	sodium acetate	0.1 (M)