1EV2
CRYSTAL STRUCTURE OF FGF2 IN COMPLEX WITH THE EXTRACELLULAR LIGAND BINDING DOMAIN OF FGF RECEPTOR 2 (FGFR2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-09-22 |
| Detector | SDMS |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.198, 71.677, 90.920 |
| Unit cell angles | 90.53, 89.98, 89.99 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.27300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.782 * |
| Data reduction software | SDMS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.042 | 0.241 |
| Total number of observations | 206913 * | |
| Number of reflections | 93440 * | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 96.3 | 87.8 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 298 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 10-15 (%) | |
| 5 | 1 | reservoir | isopropanol | 10 (%) | |
| 6 | 1 | reservoir | HEPES-NaOH | 0.1 (M) |
