1ERN

NATIVE STRUCTURE OF THE EXTRACELLULAR DOMAIN OF ERYTHROPOIETIN (EPO) RECEPTOR [EBP]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	160
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	38.760, 55.680, 58.430
Unit cell angles	62.65, 88.31, 74.97

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.216 *
Rwork	0.216
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ebp
RMSD bond length	0.008
RMSD bond angle	24.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.059 *	0.280 *
Number of reflections	14951
<I/σ(I)>	9.3	2.1
Completeness [%]	98.0	97
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.8 *	22.5 *	pH 7.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.84-0.9 (M)
2	1	reservoir	imidazole	0.1 (M)