1EQP

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1993-05-12
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	60.100, 65.300, 96.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.154
Rwork	0.156
R-free	0.19500
RMSD bond length	0.013
RMSD bond angle	0.025
Data reduction software	ROTAVATA
Data scaling software	CCP4 ((AGROVATA)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	2.010
High resolution limit [Å]	1.900 *	1.900
Rmerge	0.035	0.103
Number of reflections	26304
<I/σ(I)>	14.2
Completeness [%]	90.3	56.6 *
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	291	Chambers, R.S., (1993) J. Gen. Microbiol., 139, 325., Chambers, R.S., (1993) FEBS Lett., 327, 366. *