1EKU

CRYSTAL STRUCTURE OF A BIOLOGICALLY ACTIVE SINGLE CHAIN MUTANT OF HUMAN IFN-GAMMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-12
Detector	RIGAKU RAXIS
Spacegroup name	H 3 2
Unit cell lengths	111.380, 111.380, 311.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.246 *
Rwork	0.246
R-free	0.26500
RMSD bond length	0.008
RMSD bond angle	18.900 *
Data scaling software	SCALEPACK
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.065	0.600
Total number of observations	182491 *
Number of reflections	16757
<I/σ(I)>	25.1
Completeness [%]	99.9	100
Redundancy	10.9	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.4 M NaCitrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop	benzamidine	5 (mM)
4	1	reservoir	sodium citrate	1.4 (M)
5	1	reservoir	HEPES	100 (mM)