1EKU
CRYSTAL STRUCTURE OF A BIOLOGICALLY ACTIVE SINGLE CHAIN MUTANT OF HUMAN IFN-GAMMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-12 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 111.380, 111.380, 311.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.246 * |
| Rwork | 0.246 |
| R-free | 0.26500 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 18.900 * |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.065 | 0.600 |
| Total number of observations | 182491 * | |
| Number of reflections | 16757 | |
| <I/σ(I)> | 25.1 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.9 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.4 M NaCitrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | benzamidine | 5 (mM) | |
| 4 | 1 | reservoir | sodium citrate | 1.4 (M) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) |
