1EK5

STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE IN COMPLEX WITH NAD+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	273
Detector technology	AREA DETECTOR
Collection date	1999-05-27
Detector	SIEMENS HI-STAR
Spacegroup name	C 2 2 21
Unit cell lengths	50.300, 116.400, 130.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.191
Rwork	0.190
R-free	0.22300
RMSD bond length	0.011
RMSD bond angle	2.360
Data scaling software	SAINT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.258
Number of reflections	34184	3761 *
<I/σ(I)>	16.5
Completeness [%]	95.2	87
Redundancy	2.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG-8000, KCl, HEPPS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	18-20 (%)
2	1	reservoir	HEPPS	100 (mM)	pH7.5
3	1	reservoir		250 (mM)
4	1	drop	protein	17 (mg/ml)
5	1	drop	NAD+	2 (mM)