1EJ6
Reovirus core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 273 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-01 |
Detector | FUJI |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 1255.000, 1255.000, 1255.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.600 |
R-factor | 0.2081 |
Rwork | 0.206 |
R-free | 0.20810 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 3.710 |
High resolution limit [Å] | 3.600 | 3.470 |
Rmerge | 0.260 | 0.530 |
Number of reflections | 928767 | |
<I/σ(I)> | 2.7 | |
Completeness [%] | 98.0 | 70 |
Redundancy | 5.8 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 298 | sodium chloride, sodium acetate, magnesium chloride, hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | 0.5 (M) | ||
3 | 1 | drop | 0-0.3 (M) | ||
4 | 1 | drop | 50 (mM) | ||
5 | 1 | drop | HEPES | 50 (mM) | |
6 | 1 | reservoir | 1 (M) | ||
7 | 1 | reservoir | 0-0.6 (M) | ||
8 | 1 | reservoir | HEPES | 100 (mM) |