1EIN
THE STRUCTURAL ORIGINS OF INTERFACIAL ACTIVATION IN THERMOMYCES (HUMICOLA) LANUGINOSA LIPASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-01 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 65 |
| Unit cell lengths | 135.950, 135.950, 149.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.400 - 3.000 |
| Rwork | 0.210 |
| R-free | 0.24100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.029 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.400 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.200 | 0.425 * |
| Number of reflections | 31460 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 99.5 * | 98.2 * |
| Redundancy | 5.2 | 2.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.1 | PEG 5000, magnesium chloride, c8e5 , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 110K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 4 | 1 | reservoir | 25 (mM) | ||
| 5 | 1 | reservoir | mPEG5000 | 17 (%(w/v)) |
