1EGN

CELLOBIOHYDROLASE CEL7A (E223S, A224H, L225V, T226A, D262G) MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-30
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	83.110, 83.340, 110.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.24
Rwork	0.240
R-free	0.27000
RMSD bond length	0.005
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.064	0.237
Number of reflections	50924 *
<I/σ(I)>	24.6
Completeness [%]	100.0 *	99.9
Redundancy	4.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	sodium acetate, PEG 5000 monomethyl-ether, glycerol, sodium morpholine-ethane-sulphonic acid, cobalt chloride , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.2 (mg/ml)
2	1	drop	sodium acetate	10 (mM)
3	1	reservoir	PEG5000 MME	18.75 (%)
4	1	reservoir	glycerol	12.5 (%)
5	1	reservoir	sodium MES	20 (mM)
6	1	reservoir		20 (mM)