1ECP
PURINE NUCLEOSIDE PHOSPHORYLASE
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | XUONG-HAMLIN MULTIWIRE MARK II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.500, 110.600, 74.600 |
Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.000 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.29000 |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.300 * |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.087 |
Total number of observations | 230200 * |
Number of reflections | 70600 * |
Completeness [%] | 70.0 |
Redundancy | 3.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 12-14 (%) | |
2 | 1 | reservoir | citrate | 50 (mM) |