1EAZ

Crystal structure of the phosphoinositol (3,4)-bisphosphate binding PH domain of TAPP1 from human.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-25
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	65.017, 102.796, 41.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.171
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fb8
RMSD bond length	0.010
RMSD bond angle	1.980
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.058	0.115
Number of reflections	27661
<I/σ(I)>	21.7	4.2
Completeness [%]	98.9	97.7
Redundancy	4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	20 *	0.085M SODIUM CITRATE, 25.5% PEG 4000, 15% GLYCEROL, 0.17M AMMONIUM ACETATE, pH 5.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14.5 (mg/ml)
2	1	reservoir	sodium citrate	0.085 (M)	pH5.6
3	1	reservoir	PEG4000	25.5 (%(w/v))
4	1	reservoir	glycerol	15 (%(v/v))
5	1	reservoir	ammonium acetate	0.17 (M)