1E8X
STRUCTURAL INSIGHTS INTO PHOSHOINOSITIDE 3-KINASE ENZYMATIC MECHANISM AND SIGNALLING
Replaces: 1QMMExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID2 |
Synchrotron site | ESRF |
Beamline | ID2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-05-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.288, 67.561, 106.952 |
Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.200 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.30600 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 21.800 * |
Data scaling software | SCALA |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.095 | 0.530 |
Number of reflections | 49599 | |
<I/σ(I)> | 14.3 | 1.1 |
Completeness [%] | 95.5 | 93.3 |
Redundancy | 3.9 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 * | 17 * | used hair seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 0.1 (M) | |
2 | 1 | reservoir | ammonium sulfate | 250 (mM) | |
3 | 1 | reservoir | PEG4000 | 19 (%) |