1E7R
GDP 4-keto-6-deoxy-D-mannose epimerase reductase Y136E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 103.300, 103.300, 75.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.600 |
Rwork | 0.139 |
R-free | 0.18000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bws |
RMSD bond length | 0.017 |
RMSD bond angle | 0.038 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.043 | |
Number of reflections | 61196 | |
<I/σ(I)> | 9.65 | 2.4 |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 21 * | 1.5 M LITHIUM SULPHATE, PH 6.5 0.1M TRIS BUFFER 21C |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 11-20 (mg/ml) | |
2 | 1 | 2 | lithium sulfate | 1.5 (M) | |
3 | 1 | 2 | MES | 0.1 (M) | or Tris |