1E42
Beta2-adaptin appendage domain, from clathrin adaptor AP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-15 |
| Detector | ADSC QUANTUM |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.040, 124.590, 67.640 |
| Unit cell angles | 90.00, 124.18, 90.00 |
Refinement procedure
| Resolution | 67.420 - 1.700 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.24600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 1.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.157 | 0.750 |
| Number of reflections | 71562 | |
| <I/σ(I)> | 11.2 | 1.48 |
| Completeness [%] | 98.4 | 98.3 * |
| Redundancy | 6.85 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.8 | WELL SOLUTION: 1.6 - 2.0M MGCL2, 0.1M BICINE PH 8.7 - 9.0, 15% GLYCEROL, 1MM DTT. PROTEIN: 40MG/ML, 5MM HEPES, 50MM NACL, 4MM DTT, 1:1 MIXTURE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 5 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 4 (mM) | |
| 4 | 1 | drop | protein | 40 (mg/ml) | |
| 5 | 1 | reservoir | 1.6-2.0 (M) | ||
| 6 | 1 | reservoir | Bicine | 0.1 (M) | pH8.7-9.0 |
| 7 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 8 | 1 | reservoir | dithiothreitol | 10 (mM) |
