1E3U

MAD structure of OXA10 class D beta-lactamase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-15
Detector	MARRESEARCH
Wavelength(s)	0.95375, 1.0376
Spacegroup name	P 1 21 1
Unit cell lengths	65.530, 82.940, 101.420
Unit cell angles	90.00, 95.03, 90.00

Refinement procedure

Resolution	40.000 - 1.660
R-factor	0.1806 *
Rwork	0.181
R-free	0.20966
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.016
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.100	1.750
High resolution limit [Å]	1.653	1.650
Rmerge	0.064 *	0.322 *
Total number of observations	598857 *
Number of reflections	127968
<I/σ(I)>	8.1	2.1
Completeness [%]	98.9	98.9 *
Redundancy	4.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	4 *	AMMONIUMS SULFATE 2.0 M, TRIS HCL 100 MM, PH 8.2-8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium potassium phosphate	20 (mM)
3	1	reservoir	ammonium sulfate	2.2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)