1E2X
FadR, fatty acid responsive transcription factor from E. coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9535, 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.711, 101.973, 87.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.480 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23700 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 19.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.448 |
| Number of reflections | 18125 | |
| <I/σ(I)> | 9.7 | 2.6 |
| Completeness [%] | 95.2 | 73.8 * |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | LI2SO4/(NH4)2SO4, TRIS PH 9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.6 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.3 (M) | |
| 5 | 1 | reservoir | 0.75 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
