1DZA

3-D structure of a HP-RNase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	27.850, 67.420, 114.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 1.650
R-factor	0.177
Rwork	0.185
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rsa
RMSD bond length	0.009
RMSD bond angle	0.026
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.133	0.337
Total number of observations	80829 *
Number of reflections	25881
<I/σ(I)>	3.3	2.9
Completeness [%]	96.6	63.2
Redundancy	3.1	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		10 (mg/ml)
2	1	reservoir	PEG4000	30-35 (%(v/v))
3	1	reservoir	Tris-HCl	0.1 (M)