1DYS

Endoglucanase CEL6B from Humicola insolens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-10-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	109.917, 104.451, 53.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.600
R-factor	0.18 *
Rwork	0.180
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRICHODERMA REESEI CELLOBIOHYDROLASE II (T A JONES PERSONAL COMMUNICATION)
RMSD bond length	0.012
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.065	0.317
Number of reflections	78038
<I/σ(I)>	17	3.6
Completeness [%]	98.9	98 *
Redundancy	3.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *		THE PROTEIN (20 MG/ML-1) WAS CRYSTALLISED FROM 30% (W/V) PEG 4000K IN 10MM TRIS-ACETATE BUFFER AT PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	PEG4000	25 (%(w/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)