1DSZ
STRUCTURE OF THE RXR/RAR DNA-BINDING DOMAIN HETERODIMER IN COMPLEX WITH THE RETINOIC ACID RESPONSE ELEMENT DR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 113 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-05-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.660, 33.900, 101.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26700 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.071 | 0.170 |
| Number of reflections | 29834 | |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 94.8 | 98 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 8 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris buffer | ||
| 2 | 1 | 1 | NH4Cl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | PEG 3350 | ||
| 5 | 1 | 2 | PEG 3350 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.90 (mM) | |
| 2 | 1 | drop | DNA | 0.45 (mM) | |
| 3 | 1 | drop | Tris-HCl | 25 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 18-23 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 25 (mM) | |
| 6 | 1 | reservoir | 5 (mM) | ||
| 7 | 1 | reservoir | 0.4 (M) |
