1DSY

C2 DOMAIN FROM PROTEIN KINASE C (ALPHA) COMPLEXED WITH CA2+ AND PHOSPHATIDYLSERINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-02-25
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	58.800, 58.800, 91.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.000 - 2.600
Rwork	0.195
R-free	0.23300 *
RMSD bond length	0.015 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.092	0.281
Total number of observations	17072 *
Number of reflections	5610
<I/σ(I)>	9.4
Completeness [%]	94.3	95.1
Redundancy	2.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	20 *	PEG8K, POTASSIUM PHOSPHATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4-8 (mg/ml)
2	1	drop	calcium chloride	25 (mM)
3	1	drop	DCPS	2 (mM)
4	1	drop		25 (mM)
5	1	reservoir	PEG8000	20 (%)
6	1	reservoir	potassium phosphate	50 (mM)