1DNP

STRUCTURE OF DEOXYRIBODIPYRIMIDINE PHOTOLYASE

Experimental procedure

Source type	ROTATING ANODE
Detector technology	AREA DETECTOR
Collection date	1991-10
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1
Unit cell lengths	62.600, 72.200, 58.500
Unit cell angles	99.10, 101.50, 72.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.172
Rwork	0.172
R-free	0.24600
RMSD bond length	0.012
RMSD bond angle	1.390 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.300
Rmerge	0.043
Total number of observations	76311 *
Number of reflections	33086
Completeness [%]	78.3
Redundancy	2.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.6 *		seeding. refer to Park, H.W., (1993) J.Mol.Biol., 231, 1122. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	photolyase	4 (mg/ml)
2	1	drop	glycerol	20 (%)
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	reservoir
5	1	reservoir	PEG4000	30 (%(w/v))
6	1	reservoir		0.9 (M)
7	1	reservoir	Tris-HCl	100 (mM)