1DJP

CRYSTAL STRUCTURE OF PSEUDOMONAS 7A GLUTAMINASE-ASPARAGINASE WITH THE INHIBITOR DON COVALENTLY BOUND IN THE ACTIVE SITE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-12-12
Detector	RIGAKU RAXIS IV++
Spacegroup name	C 2 2 21
Unit cell lengths	78.510, 135.910, 137.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.350 - 1.900
R-factor	0.189
Rwork	0.189
R-free	0.22800
RMSD bond length	0.007
RMSD bond angle	24.900 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.960
High resolution limit [Å]	1.900	1.900
Rmerge	0.076	0.372
Number of reflections	50192
<I/σ(I)>	37.8
Completeness [%]	85.7	83.7
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	2.0 M Ammonium Sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.0 (M)