1D6S

CRYSTAL STRUCTURE OF THE K41A MUTANT OF O-ACETYLSERINE SULFHYDRYLASE COMPLEXED IN EXTERNAL ALDIMINE LINKAGE WITH METHIONINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	96.940, 149.930, 53.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 2.300
R-factor	0.174
Rwork	0.174
R-free	0.22300
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Rmerge	0.105

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	297	30 % PEG 4000 8 % ETHANOL 100 MM TRIS (PH 7.0) 150 MM LI2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	30 (%(w/v))
2	1	reservoir	ethanol	8 (%(v/v))
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir		0.15 (M)
5	1	drop	protain	20.0 (mg/ml)