1D6A
STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN COMPLEXED WITH GUANINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-29 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.294, 49.411, 64.288 |
| Unit cell angles | 68.66, 81.04, 63.87 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.19 * |
| Rwork | 0.190 |
| R-free | 0.25000 |
| Starting model (for MR) | 1qci |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.000 * |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.061 | 0.158 |
| Number of reflections | 26047 | |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 87.5 | 80.2 |
| Redundancy | 1.71 | 1.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 16-18% PEG 4000, 0.1M CACL2, 5MM GUANINE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 22 (%) | |
| 3 | 1 | reservoir | 0.1 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 5 | 1 | reservoir | guanine | 5 (mM) |
