1D1P

CRYSTAL STRUCTURE OF A YEAST LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE (LTP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-11-12
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	41.586, 67.647, 115.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.200
R-factor	0.169
Rwork	0.169
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Bovine low molecular weight protein tyrosine phosphatase (BPTP) PDB ID: 1PNT
RMSD bond length	0.013
RMSD bond angle	1.680 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.058	0.204
Total number of observations	140622 *
Number of reflections	15614
<I/σ(I)>	25.9	7.28
Completeness [%]	91.1	84.6
Redundancy	9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	PEG 3400, HEPES, sodium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG3400	18 (%)
3	1	reservoir		100 (mM)
4	1	reservoir	HEPES	100 (mM)