1CWD

HUMAN P56LCK TYROSINE KINASE COMPLEXED WITH PHOSPHOPEPTIDE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	44.660, 46.660, 58.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.250
R-factor	0.185
Rwork	0.185
RMSD bond length	0.015
RMSD bond angle	1.850
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.250
Rmerge	0.077 *
Total number of observations	13232 *
Number of reflections	5072 *
Completeness [%]	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.05 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		10 (mM)
2	1	drop	PEG8000	10.5 (%(w/v))
3	1	drop		0.02 (%(w/v))
4	1	drop	Lck/peptide 1	2 (mM)
5	1	reservoir		20 (mM)
6	1	reservoir	PEG8000	21 (%(w/v))
7	1	reservoir		0.04 (%(w/v))