1CSB

Crystal structure of cathepsin b inhibited with CA030 at 2.1 angstroms resolution: A basis for the design of specific epoxysuccinyl inhibitors

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-11
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	86.510, 34.150, 85.530
Unit cell angles	90.00, 103.10, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.194
Rwork	0.194
RMSD bond length	0.011 *
RMSD bond angle	1.580
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.098
Number of reflections	51421
Completeness [%]	79.1	64 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.5 (M)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	drop	protein	5 (mg/ml)