1COF

YEAST COFILIN, ORTHORHOMBIC CRYSTAL FORM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1995
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	30.669, 48.773, 101.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
Rwork	0.184
Structure solution method	MIR
RMSD bond length	0.011 *
RMSD bond angle	1.610 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	26.200
High resolution limit [Å]	2.300
Rmerge	0.067 *
Number of reflections	6330
<I/σ(I)>	18.5
Completeness [%]	87.5
Redundancy	2.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2		CRYSTALLIZED FROM 25% PEG 4000 SOLUTION., pH 7.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20-30 (%)