Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.340, 80.340, 134.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.000 * - 2.800 |
| R-factor | 0.19 * |
| Rwork | 0.280 |
| R-free | 0.33600 |
| Structure solution method | SIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.300 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.122 * | 0.374 * |
| Total number of observations | 88794 * | |
| Number of reflections | 20677 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 91.6 | 89.2 * |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 15 (%) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | MES | 0.1 (M) | pH6.5-7.0 |
