1CM8
PHOSPHORYLATED MAP KINASE P38-GAMMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.500, 66.820, 206.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.232 * |
| Rwork | 0.232 |
| R-free | 0.28260 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.400 | 2.400 * |
| Rmerge | 0.067 * | |
| Total number of observations | 118429 * | |
| Number of reflections | 31732 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 89.9 | 76.5 * |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 * | pH 7.0, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 2 | 1 | drop | AMP-PNP | 5 (mM) | |
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 6 | 1 | reservoir | PEG4000 | 27 (%) | |
| 7 | 1 | reservoir | 10 (mM) | ||
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
