1CGH

Human cathepsin G

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-09-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	40.040, 63.610, 79.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.19
Rwork	0.190
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BOVINE BETA TRYPSIN (W.BODE)
RMSD bond length	0.013
RMSD bond angle	1.765
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.057	0.327 *
Total number of observations	68129 *
Number of reflections	18433
Completeness [%]	95.1	95.3 *
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	20 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	citrate	1.2 (M)