1CGH
Human cathepsin G
Experimental procedure
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-09-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.040, 63.610, 79.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.800 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | BOVINE BETA TRYPSIN (W.BODE) |
RMSD bond length | 0.013 |
RMSD bond angle | 1.765 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.800 * | 1.800 * |
Rmerge | 0.057 | 0.327 * |
Total number of observations | 68129 * | |
Number of reflections | 18433 | |
Completeness [%] | 95.1 | 95.3 * |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 20 * | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | citrate | 1.2 (M) |